Paramecium eating pigmented yeast

wxfix asked:

As the paramecium stuffs itself with a meal of pigmented yeast, vacuoles form. As food particles are digested by acidic enzymes, the congo red will reveal the pH drop-color will shift from a more alkaline red, to a more acidic blue. “They Pulled My Jazz soundtrack!” : (

Candida

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22 Comments

Ghostmonsters  on August 12th, 2010

@biotekagr ;-;

biotekagr  on August 14th, 2010

omgod your hurting that poor thing!!

duck24x  on August 15th, 2010

Perfect music to go with a great video!

therealsuperhobo  on August 17th, 2010

OMG this bacterial cruelty!!!1

argeque  on August 18th, 2010

Poor thing is getting zerg-rushed.

mynameisnottoolong  on August 20th, 2010

@welcomeToUrMooseyFat you can add Glycerol to the water it will make it thicker.

welcomeToUrMooseyFat  on August 23rd, 2010

love the music ^_^ how did you get the paramecium to stay like it did? i have only found one that did that for me!

orenco  on August 27th, 2010

Is it bad to eat pure yeast?

stevensartifacts  on August 30th, 2010

@wxfix thank you!! that’s it! rotifers!

wxfix  on August 31st, 2010

Sounds like you have some Rotifers. They tooo are fun to watch.

stevensartifacts  on September 1st, 2010

i got some Chaos (Pelomyxa) carolinensis from Carolina Bio supply— i’ve seen paramecium in the culture, but there are these other long things about the size of paramecium, that sometimes suck up food, and sometimes open their heads into too circular ‘weapons’ with super fast rotating cilia (like chainsaws) that whip food into their mouth—-

what are these things???

wxfix  on September 1st, 2010

I use a disposable plastic pipette. You could try one of those “booger bulbs” folks use on babies to suck up your protists and put a drop or two on your slide.

wxfix  on September 1st, 2010

1) I made sure i had a happy bunch of paramecium.
2) I put 1/2 envelope (1tsp or so) of regular bread yeast in 100ml warm water. Then I let it sit somewhere near 37C for a couple hours, till I saw a lot of bubbles in the center of the glass.
3) I added about a dozen or so drops of 1% congo red dye into the glass.
4)Meanwhile, I took a sampling of paramecium onto a depression slide. I waited a couple hours before I applied the congo red yeast.
5) Because it was cold, I didn’t have to.

excelsistenz  on September 2nd, 2010

can you tell me how you prepared the yeast stained with congo red… also,, did you apply something that made the paramecium move slower???

Beardactal  on September 3rd, 2010

Micro Kombat!

Gurufail  on September 4th, 2010

Thank you so much for posting this. We did a lab in biology and I didn’t quite get to this part.

RyanatorML2000  on September 4th, 2010

Is there any way to “pick up” and move these microorganisms? I’ve tried to do it several times and ended up just moving them a bit to the side or squashing them.

mrssasuke38385  on September 6th, 2010

lmao its so cute haha

bonec120  on September 8th, 2010

yes, they can move extremely fast

WRAYDAY  on September 11th, 2010

yeah in lab we slow them down with a viscous substance called detain.

CarinaSuda  on September 11th, 2010

wow really interesting. Thanks

wxfix  on September 14th, 2010

Yes. This video was shot in real time. This is not a compiled animation. In fact, Sometimes the paramecium move even faster than this.