Stop Candida

subasavage  on July 12th, 2010

Huh, it seems to come up for me. Anyone else having trouble viewing this video?

Thanks!

lumbardh  on July 12th, 2010

I can’t get this one video of yours to play. Everything else works fine. Great info so far!

dvdfrnzwbr  on July 13th, 2010

It works! Haven’t purchased the lab equipment yet. I use baby food jars, a paper clip wire, and my gas kitchen stove. The jars have autoclaving lids and what works good is that I mix the ingredients for the agar in each jar, pressure cook, and they autoclave shut. Agar forms well after cooling. To streak a new yeast, just take a jar out of storage and “pop” one open. Sterilized inside and no contaminated jars yet. Give streaking yeast a try. Looks hard but easy. Thanks Subasavage for your help!

funkmasterchilly  on July 15th, 2010

i have just watched Yeast Starter, Yeast Propogation, & Bottle Culturing Part 2 and had seen the infected plate and was like “oh I just asked a question about that” so I came back here to comment on my comment and seen you had already answered my question. that was quick. Your good

subasavage  on July 18th, 2010

I much appreciate the feedback…glad to be of help. As for leaving the plates untouched, you have heard correctly that this is a good practice. You’ll want to leave these at room temp so any possible contaminants can grow. I have found that a fair fraction do become contaminated (~10%) somehow (perhaps not a surprise without a laminar hood). Recently to save time, I have begun streaking right away and if the plate is contaminated, I’ll streak again (either way, you lose the first plate).

funkmasterchilly  on July 19th, 2010

First off thank for the great vid’s. This is the first video I’ve found on youtube laying out step by step instructions on making agar plates and cultivating yeast. I’ve been looking for one of these for a wile now B/C I think I’m going to try doing this soon. With that said one thing I’ve read is after making up the agar plates some ppl leave the plates untouched for a week or two to make sure the plates are uncontaminated before streaking. do you do this?

crazyboy0602  on July 20th, 2010

Yeast vials can keep for about 1 year probably more. One vial can be cultured about four generations. After that the yeast will mutate.

subasavage  on July 21st, 2010

Well, I’m too new to this to provide a definitive answer. I think that the yeast labs (i.e., White & Wyeast) essentially do the same thing we’re doing here (though they don’t keep slants for a year, they restreak more frequently). After a period of several years, they re-evaluate the strain to see that it still has the intended characteristics. So in effect, you should be able to re-streak a significant number of times without worry. Not the same as repitching yeast (i.e., generation +1).

dvdfrnzwbr  on July 24th, 2010

Any thoughts on how many years, or generations, you can farm a yeast? What is your opinion about perhaps buying a new liquid yeast pack from the store after a certain amount of years, or generations, and starting over again?
Thank you for your excellent video presentations, and your detailed responses. We are all forever grateful for the sharing of your knowledge!

subasavage  on July 25th, 2010

I use the petri dish of yeast as a working sample for ~6 months. After that (or if it gets contaminated), I soak it in a 10% bleach solution for a while and remove the agar. After a thorough cleaning and rinsing, it’s ready to go into the pressure cooker for sterilization. Once sterile, it’s ready for a new pouring of wort agar. After ~1 year, I’ll restreak all strains from the slants to the plates, let them grow, then streak new slants to last for another year.

dvdfrnzwbr  on July 26th, 2010

Can you shed a little light on what you do with the petri dish of yeast after you transfer the yeast colony to a slant vial. Do you clean it out, or or do you hang on to the dish culture as a working sample for future use, and dip into it every time you create a starter wort? If so, how long until you restreak a new dish? The tube slant you created for long term storage, from the dish, I assume is just for archive and regernerating new petri dishes? Am I onto the right track?

subasavage  on July 27th, 2010

These vials I use are plastic…probably best not to flame them. As for the dish, if you do not store it upside down the moisture (that you will inevitably have from condensation when pouring hot agar into the dish and letting it cool closed) will coat the top of the agar and yeast sample. Perhaps not crucial but inverting the dish prevents this. Cheers!

scion171777  on July 28th, 2010

hey, u should let flame touch the mouth of the vial if you open and close it.. anyway, great video…. does it really need to invert the position of the dish containing culture media while incubating?

subasavage  on July 30th, 2010

Would you believe I started taping a yeast culture from a Duvel bottle and it didn’t work so I decided not to post it. I’ve been looking to give it another try but haven’t found the time. Off to the NHC this weekend!

merrickne  on August 2nd, 2010

Great video but when are you going to do the video on propagation? Don’t leave me hanging!

dwalken  on August 4th, 2010

This is great stuff…look forward to the bottle culture.

bsfixit101  on August 7th, 2010

I never knew about the upside down technique
but i have a flow hood for that ..
good video thanks

jasonmeisner  on August 9th, 2010

Great video, thanks for taking the time to give this lesson!